A novel mechanism for regulating clonal propagation of mouse ES cells

Self-grafting a simple method for vegetative propagation of apple clonal rootstocks

Inducible Transgene Expression in Mouse ES Cells

Embryonic stem (ES) cells serve as a potentially unlimited source of cells and tissues to treat a number of genetic and malignant diseases. The differentiation of these cells into specific cell types is an area of very active investigation. One method of manipulating ES cell differentiation is through the alteration of gene expression. There are a multitude of different methods for expressing a...

Targeted gene trapping in mouse ES cells

Roland Friedel et al. report a method allowing efficient generation of targeted gene knockouts by homologous recombination in mouse ES cells. Current gene targeting techniques use vectors with a strong promoter to drive expression of a selectable antibiotic resistance marker, but such methods usually yield low homologous recombination frequencies because nonhomologous insertions (false positive...

A vector-based system for the differentiation of mouse embryonic stem cells toward germ-line cells

Objective(s):To culture thein vitro mouse embryonic stem cells (mESCs) and to direct their  differentiation to germ-line cells; in present study we used a vector backbone containing the fusion construct Stra8-EGFP to select differentiated ES cells that entered meiosis.  Retinoic acid was used to differentiate embryonic stem cells to germ cells. Materials and Methods: A fragment of Stra8 gene pr...

wuthering heights and the concept of marality/a sociological study of the novel

to discuss my point, i have collected quite a number of articles, anthologies, and books about "wuthering heights" applying various ideas and theories to this fantastic story. hence, i have come to believe that gadamer and jauss are rightful when they claim that "the individaul human mind is the center and origin of all meaning," 3 that reading literature is a reader-oriented activity, that it ...